As basal cells of stratified squamous epithelia begin to migrate in response to wound healing, they lose their cell-substrate adhesion junctions, the hemidesmosomes.
The hemidesmosome is an adhesion structure of the epidermal-dermal junction in keratinocytes.
When keratinocytes migrate laterally or upward to differentiate they must control the formation and disintegration of the hemidesmosomes.
The bullous pemphigoid antigen BPAG1 is a hemidesmosomal protein of the cutaneous basement membrane zone.
The primary sequence deduced from full-length human cDNAs predicts that this molecule consists of a central rod region and flanking globular domains.
A neuronal isoform, BPAG1n3 is the result of differential splicing of BPAG1.
BPAG1n3 is distinguished by its initial 32 amino acid residues and by the absence of the amino-terminal half of the actin-binding domain.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of DST. Species Crossreactivity: Human, mouse, rat
