Desmogleins (dsgs) are type I membrane proteins that are important for cell adhesion and are expressed in great abundance at the desmosomes, which are adhesive cell junctions.
The dsg proteins belong to the cadherin family and consist of dsg1, dsg2 and dsg3.
Calcium binds to the putative calcium binding sites at the extracellular N-terminal domain of dsg1, which has cadherin- like repeats.
Unlike normal human keratinocytes, the squamous cell carcinoma cells exhibit diminished or unusual expression of dsg3 and dsg1, which bear pemphigus vulgaris and pemphigus foliaceus antigens, respectively.
Cultured normal human keratinocytes express dsg1 and dsg3 mRNA, with or without dsg2 mRNA, which indicates that desmoglein isoforms exhibit abnormal expression and may be related to tumor cell kinetics, such as cell invasion and metastasis.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Dsg1. Species Crossreactivity: Human, mouse
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to amino acids 170-220 of Human Dsg1.
