84-2325-49　［取扱停止］DPYD (Dihydropyrimidine Dehydrogenase [NADP(+)], DHPDHase, DPD, Dihydrothymine Dehydrogenase, Dihydrouracil Dehydrogenase, DPYD) 50ul　222230

特徴

• Dihydropyrimidine dehydrogenase (DPD, DPYD) catalyzes the initial and rate-limiting step in uracil and thymidine catabolism as well as catabolism of the chemotherapeutic drug 5-fluorouracil (5-FU) and its derivatives.
• DPYD deficiency, which results from mutations in the DPYD gene, causes errors in pyrimidine metabolism and potentially life-threatening side effects in cancer patients treated with 5-FU (reviewed in 1).
• As a result, ongoing work examines whether or how DPYD gene variation and protein expression can be used to predict 5-FU toxicity.
• Several genes that impart resistance to 5-FU were recently identified in human hepatocellular carcinoma (HCC).
• AEG-1, which is highly expressed in HCC, increases the expression of DPYD.
• DPYD is expressed more highly in HCC than in normal liver, and this is thought to be one mechanism of 5-FU resistance.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.