84-2325-06　［取扱停止］DNMT3A (DNA (cytosine 5) Methyltransferase 3 alpha, DNA (cytosine 5) Methyltransferase 3 alpha, DNA (cytosine 5) Methyltransferase 3A, DNA (cytosine-5)-Methyltransferase 3A, DNA cytosine Methyltransferase 3A2, DNA Methyltransferase 3a, DNA Methyltransferase 3a, DNA Methyltransferase HsaIIIA, DNA MTase HsaI, DNA MTase HsaI, DNA MTase HsaIIIA, DNM3A_HUMAN, DNMT 3a, ...　222208

特徴

• Methylation at the 5'-position of cytosine is the only known naturally occurring covalent modification of the mammalian genome.
• DNA methylation requires the enzymatic activity of DNA 5-cytosine methyltransferase (Dnmt) proteins, which catalyze the transfer of a methyl group from S-adenosyl methionine to the 5'-position of cytosines residing in the dinucleotide CpG motif, and this methylation results in transcriptional repression of the target gene.
• The Dnmt enzymes are encoded by independent genes.
• Dnmt1 is the most abundant, and it preferentially methylates hemimethylated DNA and coordinates gene expression during development.
• Additional mammalian Dnmt proteins include Dnmt2 and Dnmt3.
• Dnmt2 lacks the large N-terminal regulator domain of Dnmt1, is expressed at substantially lower levels in adult tissues, and is likely involved in methylating newly integrated retroviral DNA.
• Dnmt3a and Dnmt3b are encoded by two distinct genes, but both are abundantly expressed in embryonic stem cells, where they also methylate CpG motifs on DNA.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.