Internucleosomal DNA fragmentation following the activation of endonucleases is the common end point of apoptosis.
DNase I, a Ca2+/Mg2+-dependent endonuclease ubiquitously expressed in mammalian tissues, has been implicated to mediate internucleosomal DNA degradation in human cells undergoing apoptosis.
DNase I is highly polymorphic, and at least six alleles of DNase I are known.
DNase II, the ubiquitously expressed acidic deoxyribonuclease, acts downstream of caspase activation and may also induce DNA digestion during apoptosis.
DNase I cleaves DNA to 5'-phosphodinucleotide and 5'-phospho- oligonucleotide end-products, whereas DNase II cleaves DNA to 3'-phosphomononucleotide and 3'-phosphooligonucleotide end-products.
The mechanism by which DNase II cuts DNA is similar to DNase I, which produces nicks rather than double-strand cuts.
DNase II is usually present in cytoplasm of epithelial cells, but it appears concentrated in the nuclei of lens fibers.
In contrast, DNase I is always concentrated in nuclei of epithelial and fiber cells.
Applications:Suitable for use in Western Blot and Immunofluorescence/Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunofluorescence/Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human DNASE1. Species Crossreactivity: mouse, rat
