84-2324-71　［取扱停止］DNA Polymerase epsilon 2 (DNA Polymerase epsilon Subunit 2, DNA Polymerase II Subunit 2, DNA Polymerase epsilon Subunit B, POLE2, DPE2, DNA pol ε2, DNA pol εB, DNA pol ε B, DNA polε2, DNApolε2, DNA polεB, DNApolεB) 100ul　222190

特徴

• DNA replication, recombination and repair, all of which are necessary for genome stability, require the presence of exonucleases.
• In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair, they function to excise damaged DNA fragments and correct recombinational mismatches.
• Exonucleases involved in these processes include DNA polymerases, including DNA pol delta and ε.
• DNA pol delta consists of two subunits-p125 which interacts directly with the sliding DNA clamp protein PCNA, and p50.
• DNA pol delta can be regulated by cell cycle proteins.
• DNA pol ε is a multiple subunit enzyme, the catalytic subunit of which is encoded by the POL2 gene.
• The exact reactions catalyzed by DNA pol delta and ε on leading and lagging strands have not yet been elucidated.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.