84-2324-59　［取扱停止］DNA Polymerase (DNA directed DNA Polymerase beta, DNA pol beta, DNA Polymerase beta, DNA Polymerase beta Subunit, DPOLB, MGC125976, Pol B, Pol beta, PolB, Polymerase (DNA directed) beta) 100ul　222184

特徴

• DNA replication, recombination and repair, all of which are necessary for genomic stability, require the presence of exonucleases.
• In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair they function to excise damaged DNA fragments and correct recombinational mismatches.
• These exonucleases include the family of DNA polymerases.
• DNA pol alpha, beta, delta and ε are involved in DNA replication and repair.
• DNA pol delta and DNA pol ε are multisubunit enzymes, with DNA pol delta consisting of two subunits—p125, which interacts with the sliding DNA clamp protein PCNA, and p50.
• The nuclear-encoded DNA pol y is the only DNA polymerase required for the replication of the mitochondrial DNA.
• DNA pol zeta is ubiquitously expressed in various tissues and mediates the cellular mechanism of damage-induced mutagenesis.
• DNA pol θ is a DNA polymerase-helicase that binds ATP and is involved in the repair of interstrand crosslinks.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.