84-2324-55　［取扱停止］DNA Ligase I (DNA Ligase 1, DNA Ligase I, DNLI1, LIG 1, lig1, Ligase I DNA ATP Dependent, Polydeoxyribonucleotide Synthase [ATP] 1) 100ul　222182

特徴

• Eukaryotic DNA ligases are ATP-dependent enzymes that catalyse the joining of single and double-strand DNA breaks, which is an essential final step in DNA replication, recombination and repair.
• Four biochemically distinct DNA ligases, termed ligases I-IV, have been identified in mammalian cells.
• DNA ligase I is functionally homologous to the DNA ligase encoded by the Saccharomyces cerevisiae Cdc9 gene.
• The joining of Okazaki fragments during lagging strand DNA replication in mammalian cells is due to DNA ligase I.
• A combination of DNA polymerase epsilon, PCNA, replication factor C, replication protein A, and DNA ligase I is well-suited to the task of creating nucleotide excision repair patches.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.