84-2324-52　［取扱停止］DNA Ligase (DNA Ligase IV ATP Dependent, DNA joinase, DNA Ligase 4, DNA Ligase IV, DNA Repair Enzyme, DNLI4, LIG 4, LIG4, Ligase IV, Ligase IV DNA ATP Dependent, Polydeoxyribonucleotide Synthase [ATP] 4, Polydeoxyribonucleotide Synthase 4, Polydeoxyribonucleotide Synthase, Polynucleotide Ligase, Sealase) 50ul　222181

特徴

• The X-ray repair cross-complementing protein XRCC4 and DNA Ligase IV are essential for repairing double-strand breaks in DNA.
• These proteins form a critical complex consisting of two molecules of each protein that preferentially bind DNA with nicks or broken ends.
• As an obligate accessory molecule, XRCC4 binds to DNA Ligase IV and enhances its joining activity.
• The XRCC4/ DNA Ligase IV complex is also involved in V(D)J recombination.
• V(D)J recombination occurs in normal development of the adaptive immune system and involves the formation of a double-strand break intermediate.
• Deletions of either DNA Ligase IV or XRCC4 inhibit the completion of V(D)J recombination, resulting in a high incidence of apoptosis in the developing nervous system and a block in B and T cell maturation.
• Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.