The “Disrupted In Schizophrenia” gene locus DISC is associated with patients afflicted with schizophrenia as a result of chromosomal translocations.
DISC-1 encodes a large protein predicted to contain a globular N-terminal domain and a helical C-terminal domain, both of which have the potential to form interactions with other proteins.
DISC-1 interacts with proteins involved in the centrosome and cytoskeletal system, including MIPT3, MAP1A and nudel; proteins which localize receptors to membranes, including a-actinin-2 and spectrin b IV; and proteins which transduce signals from membrane receptors, including ATF-4 and ATF-5.
Therefore, DISC-1 is thought to be involved in intracellular transport, neurite architecture and/or neuronal migration, all of which are thought to be pathogenic in the schizophrenic brain.
DISC-1 localizes to the nucleus, whereas mutant DISC-1 localization occurs mainly in the cytoplasm.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of DISC1. Species Crossreactivity: Human, mouse, rat
