Dio-1 (Death Inducer-Obliterator-1) is a putative transcription factor that contains two zinc finger motifs.
Dio-1 translocates to the nucleus, and activates apoptosis during limb development.
Programmed cell death, a highly regulated form of apoptosis, plays an important role in determining the amount of tissue, the shape, and the definition of each digit during limb development.
Dio-1 expression is upregulated when an apoptotic signal is detected, and subsequently apoptosis is induced.
This process is similar to the expression of NFκB and NGF in response to external signals.
Dio-1 expression is suppressed by caspase inhibitors and Bcl-2 expression.
This supports the theory that Dio-1 functions in the onset of programmed cell death.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Dio-1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 154-198 of Human Dio-1.
