Deoxyhypusine synthase (DHS) is crucial for the posttranslational formation of hypusine, a modification of a specific lysine residue in eukaryotic initiation factor 5A (eIF-5A).
Hypusine is formed by posttranslational modifications involving two enzymatic steps catalyzed by DHS and deoxyhypusine hydroxylase (DOHH).
eIF-5A is essential for eukaryotic cell proliferation.
DHS, which belongs to the deoxyhypusine synthase family of proteins, is important for the first step in the hypusine biosynthesis pathway.
It acts as a catalyst for the NAD-dependent oxidative cleavage of spermidine and the ensuing transfer of the butylamine moiety of spermidine to the eIF-5A protein, to create the intermediate deoxyhypusine residue.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of DHS. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 50-100 of Human DHS.
