84-2323-89　［取扱停止］DGK-delta (Diacylglycerol Kinase delta, DAG Kinase delta, 130kD diacylglycerol Kinase, Diglyceride Kinase delta, DGK-delta, DGKD, KIAA0145, DGKdelta, DGK delta) 50ul　222149

特徴

• Diacylglycerol kinases (DGKs) phosphorylate diacylglycerol (DAG) to produce phosphatidic acid.
• DAG and phosphatidic acid are lipids that act as second messengers in signaling cascades.
• DGK-alpha influences cell activation and secretion of lethal exosomes, which in turn control cell death.
• DGK-beta is abundant in restricted brain regions such as the caudate putamen and olfactory tubercle.
• DGK-y encodes full-length and truncated transcripts that are present in a range of human tissues, with greatest expression observed in retina.
• DGK-delta is most abundant in skeletal muscle.
• DGK-ε shows specificity for arachidonylcontaining diacylglycerol and is expressed predominantly in testis.
• DGK-θ is most abundant in the cerebellum and hippocampus.
• DGK-ι is present in brain and retina as a predominant transcript of more than 12 kb, including a long 3-prime untranslated region, with additional low abundance transcripts of 9.5 and 7.5 kb.
• DGK-η is closely related to DGK-delta.
• DGK-zeta is most abundant in brain and muscle.
• DGKs have structural motifs that play regulatory roles, and these motifs form the basis for dividing the DGKs into five subtypes.
• Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.