84-2323-77　［取扱停止］Desmoglein 3 (Desmoglein-3, Desmoglein3, 130kD pemphigus vulgaris Antigen, PVA, Cadherin Family Member 6, DSG3, CDHF6) 50ul　222143

特徴

• Pemphigus is an autoimmune disease of skin adhesion associated with auto-antibodies against a number of keratinocyte antigens, such as the adhesion molecules desmoglein (dsg) 1 and 3 and acetylcholine receptors.
• Desmogleins, Type I membrane proteins, are important for cell adhesion and are expressed in great abundance at the desmosomes, which are adhesive cell junctions.
• Desmogleins belong to the cadherin family and consist of dsg1, dsg2 and dsg3.
• Calcium binds to the putative calcium binding sites at the extracellular N-terminal domain, which has cadherin-like repeats.
• Unlike normal human keratinocytes, squamous cell carcinoma cells exhibit diminished or un-usual expression of dsg3 and dsg1, which bear pemphigus vulgaris and pemphigus foliaceus antigens, respectively.
• Several carcinoma cell lines constantly express dsg2 and dsg3 mRNA, whereas cultured normal human keratinocytes always express dsg1 and dsg3 mRNA, with or without dsg2 mRNA.
• This expression pattern indicates that desmoglein isoforms exhibit abnormal expression and may be related to tumor cell kinetics, such as cell invasion and metastasis.
• dsg2 is the fundamental dsg common to all desmosome-possessing tissues and is the largest desmoglein in the family.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.