beta-defensins (also designated BD, and hBD in human) are small cationic peptides with broad-spectrum antimicrobial activity.
Produced in mucosal epithelia and neutrophils of several species, beta-defensins are developmentally regulated.
Unlike the other previously described human beta-defensins, human beta-defensin 4 (hBD-4) expression is restricted to a few tissues, with highest expression in testis.
A restricted pattern is also exhibited by mouse beta-defensin 4.
Rat beta-defensin 4 (also desiganated BD-4, RBD-4, BD-2, and RBD-2) is developmentally regulated in the lung and is predominantly expressed in the lung and, to a lesser extent, in the trachea and tongue.
It exhibits a regulation pattern similar to that of specific genes involved in host defense around the time of birth.
The selectivity in both expression pattern and antimicrobial activity of human beta-defensin 4 suggests that it is best suited to act at the epithelial locations where it is expressed.
Applications:Suitable for use in Western Blot, Immunoflourescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (Paraffin): 1:50-1:200Immunoflourescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human DEFB4.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to the full length human DEFB4. Swiss/UniProt Accession: O15263
