Apoptosis is a physiological process by which multicellular organisms eliminate unwanted cells.
DEDD (Death Effector Domain-containing DNA binding protein) induces apoptosis by triggering a series of intracellular protein-protein interactions mediated by the N-terminal DED motif.
DEDD, a cytoplasmic protein, translocates to the nucleus during CD95-mediated apoptosis, where it localizes to nucleoli-like structures, activates caspase-6 and specifically inhibits RNA polymerase I-dependent transcription.
The cell death activity of DEDD relates to its nuclear localization.
The DED in DEDD is sufficient for its DNA binding, capspase-6 activating and Pol I specific transcriptional repressor activity.
Point specific mutations indicate that the DED in DEDD represents a novel domain that is structually similar to other DEDs but functionally different from classical DEDs found in FADD or caspase-8.
DEDD is widely expressed in a variety of tissues, with highest levels in the testis.
The human DEDD gene maps to chromosome 1q23.3.
Alternative splicing results in two transcript variants which encode the same protein.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of DEDD. Species Crossreactivity: Human, mouse, rat
