The innate immune system senses viral infection by recognizing many different viral components and triggering specific antiviral responses.
Intracellular double-stranded RNA (dsRNA) is a major sign of replication for many viruses.
Retinoic acid inducible gene I (RIG-I) is a 925 amino acid, interferon-inducible cellular DExD/H box RNA helicase that activates type I interferon (IFN), an important effector of the innate immune system that is sensitive to these dsRNA viruses.
dsRNA is normally present in very low quantities in cells, so when a virus is present, the elevated levels of dsRNA act as a sign telling RIG-I to activate the production of IFN.
RIG-I does this by using its helicase domain to bind to viral dsRNA, thus transmitting the activation signal for IFN through IkB kinase-related kinases and inducing IFN expression.
RIG-I is expressed in the cytoplasm of fibroblasts and conventional dendritic cells and can distinguish between many different RNA viruses.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of DDX58. Species Crossreactivity: Human
