DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases.
DEAD box proteins contain two conserved RecA-like domains that adopt different open structures in the absence of nucleic acid and closed structure when bound to RNA and ATP.
They also exhibit RNA-dependent ATPase and ATP-dependent RNA-unwinding activities.
DEAD box RNA helicase DEAD5 (Dbp5), also known as DEAD box protein 19B (DDX19B), is a 479 amino acid protein belonging to the DEAD box family.
Localized to the cytoplasm and nuclear envelope, Dbp5 participates in the export of mRNA from the nucleus to the cytoplasm.
Dbp5 is activated by interactions mediated by Gle1 and is inhibited by Nup214.
Two named isoforms of Dbp5 exist as a result of alternative splicing events.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of DDX19B. Species Crossreactivity: Human, mouse
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to amino acids 6-52 of Human DDX19B.
