Tumor necrosis factor (TNF) is a pleiotropic cytokine whose function is mediated by two distinct cell surface receptors, designated TNF-R1 and TNF-R2, which are expressed on most cell types.
TNF function is primarily mediated through TNF-R1 signaling.
Both receptors belong to the growing TNF receptor superfamily which includes FAS antigen and CD40.
TNF-R1 contains a cytoplasmic motif, termed the “death domain,” that has been found to be necessary for the transduction of the apoptotic signal.
The death domain is also found in several other receptors, including FAS, DR2 (or TRUNDD), DR3 (death receptor 3), DR4, DR5 and DR6.
TRUNDD, DR4 and DR5 are receptors for the apoptosis-inducing cytokine TRAIL.
Non-death domain-containing receptors, designated decoy receptor 1 (DcR1) or TRID, DcR2 and DcR3, associate with specific ligands and may play a role in cellular resistance to apoptotic stimuli.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunofluorescence: 1:50-1:200Western Blot: 1:500-1:1000Immunohistochemistry (Paraffin): 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of DcR3 at 33kD. Species Crossreactivity: human, mouse and rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa261-310 from human DcR3 (O95407).
