The dCK/dGK family of proteins includes 4 different deoxyribonucleoside kinases including the cytoplasmic (TK1) and mitochondrial (TK2) thymidine kinases, and the deoxycytidine (dCK) and deoxyguanosine (dGK) kinases.
Deoxyribonucleoside kinases catalyze the 5’-phosphorylation of 2’-deoxyribonucleosides with nucleoside triphosphates (NTPs) as phosphate donors.
The dCK enzyme is associated with drug resistance and sensitivity, as both dCK and TK2 phosphorylate several antiviral and chemotherapeutic nucleoside analogs.
Deficiency of dCK activity corresponds with resistance to antiviral and chemotherapeutic agents.
dCK and TK1 localize to the cytosol, whereas dCK and TK2 localize to the mitochondria.
These deoxyribonucleoside kinases are most abundantly expressed in muscle, brain and liver.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of DCK. Species Crossreactivity: Human, mouse, rat
