DCC (deleted in colorectal cancer) was first identified as a candidate tumor suppressor gene based on its absence or reduced expression in the majority of colorectal cancers.
Loss of DCC expression was subsequently observed in cancers of the breast, endometrium, brain, pancreas and prostate, as well as in leukemias, neuroblastomas and male germ cell cancers.
DCC is a 1447 amino acid transmembrane protein with highest expression in developing brain and neural tube and is suspected to play a role in mediating directional migration in the developing nervous system.
Netrin-1, a chemoattractant for commissural axons in the spinal cord, has been identified as a ligand for DCC.
Applications:Suitable for use in Immunofluorescence, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of DCC. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 450-500 of Human DCC.
