Dopamine beta-hydroxylase (DBH) catalyzes the conversion of dopamine to noradrenaline in the biosynthesis of catecholamines.
DBH is selectively expressed in noradrenergic and adrenergic neurons, as well as in neuroendocrine cells, and it serves as a specific protein marker for noradrenergic processes.
The active form of DBH is a homotetramer, which is found in the lumen of synaptic vesicles of corresponding nerve cells, where it localizes to both the membrane and cytosol.
DBH is induced by nerve growth factor and insulin growth factor-1 and is regulated by intracellular second messengers protein kinase A, cyclic AMP, diacyl glycerol and Ca2+.
Expression of DBH is transcriptionally mediated by Sp1, CREB and AP-1 proteins including c-Fos, c-Jun and JunD.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of DBH. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 445-490 of Human DBH.
