DAP (death associated protein) kinase and ZIP kinase are members of a novel protein kinase family, the members of which have the capacity to mediate apoptosis through their catalytic activities.
DAP kinase (DAPK) contains a “death domain” and has been shown to mediate IFN-y-induced apoptosis.
The introduction of DAPK into highly metastatic carcinoma clones lacking DAPK expression has been shown to result in the suppression of metastasis, thus linking suppression of apoptosis to metastasis.
ZIP kinase contains a leucine zipper domain, which is necessary for homodimerization and for interaction with other leucine zipper proteins.
ZIP kinase dimerizes with ATF-4, an ATF/CREB transcription factor family member that contains a leucine zipper.
Overexpression of ZIP kinase has been shown to result in morphological changes associated with apoptosis in NIH/3T3 cells.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of DAPK1 at ~160kD. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to full length human DAPK1
