Death-associated protein 5 (DAP-5) (also known as p97 and NAT1) is a member of the eukaryotic translation initiation factor 4G (elF4G) family.
DAP-5 is ubiquitously expressed and is highly conserved among species.
In response to activated FAS or p53, caspase cleaves DAP-5 at position 790 to yield a C-terminal truncated protein which is capable of forming complexes with eIF4A and eIF3.
DAP-5 has homology to the carboxy-terminal portion of eIF4G, but lacks the N-terminal region of eIF4G, which is responsible for association with the CAP binding protein eIF4E.
By forming translationally inactive complexes with eIF4A and eIF3, but not with eIF4E, DAP-5 functions as a general repressor of translation.
During apotosis, the caspase-activated DAP-5 can mediate CAP-independent translation at least from its own internal ribosome entry site, thus resulting in a positive feedback loop responsible for the continuous translation of DAP-5.
DAP-5 is also required for cellular differentiation, as it controls specific gene expression pathways.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of DAP-5. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 35-80 of Human DAP-5.
