In contrast to growth factors which promote cell proliferation, FAS ligand (FAS-L) and the tumor necrosis factors (TNFs) rapidly induce apoptosis.
Cellular response to FAS-L and TNF is mediated by structurally related receptors containing a conserved cytoplasmic region called the “death domain”.
DAP-1 (for death associated protein-1) interacts with the death domain of TNF-R1 and can trigger programmed cell death in a variety of cell lines, as well as suppress NFκB/Rel activity.
DAP-3 is a positive mediator of programmed cell death; overexpressed intact full-length protein is required in order to induce apoptosis.
During apotosis, the caspase-activated DAP-5 can mediate CAP-independent translation at least from its own internal ribosome entry site, thus resulting in a positive feedback loop responsible for the continuous translation of DAP-5.
DAP-5 is also required for cellular differentiation, as it controls specific gene expression pathways.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of DAP. Species Crossreactivity: Human
