Cytokeratins are a subfamily of intermediate filament keratins that are characterized by a remarkable biochemical diversity, which is represented in human epithelial tissues by at least 20 different polypeptides.
Cytokeratins range in isoelectric range between 4.9 and 7.8.
Cytokeratin 1 has the highest molecular weight, while Cytokeratin 19 has the lowest molecular weight.
The cytokeratins are divided into the type I and type II subgroups.
Type II family members comprise the basic to neutral members, Cytokeratins 1-8, while the type I group comprises the acidic members, Cytokeratins 9-20.Cytokeratin 4 is expressed in differentiated layers of the mucosal and esophageal epithelia along with Cytokeratin 13.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-2000Immunohistochemistry: 1:50-200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of Cytokeratin 4. Species Crossreactivity: Human, Mouse, Rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant full length Human Cytokeratin 4.
