Cytochrome P450 3A (CYP3A) genes encode monooxygenases—enzymes which catalyze drug metabolism and the synthesis of cholesterol, steroids and other lipids.
CYP3A, the most abundant p450 enzyme in human liver, is responsible for the metabolism of more than 50% of all clinical drugs.
CYP3A family members localize in organs that associate with drug disposition, including the liver, gastrointestinal tract and kidney.
The CYP3A cluster maps to gene locus 7q21.3-q22.1 and consists of four genes (CYP3A4, CYP3A5, CYP3A7 andCYP3A43) and two pseudogenes (CYP3A5P1 and CYP3A5P2).
CYP3A4 is abundant in the endoplasmic reticulum of liver cells and upper intestinal enterocytes.
CYP3A4 expression is inducible by glucocorticoids pharmacological agents.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of CYP3A4/5. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 351-400 of Human CYP3A4.
