CYP11A1, also known as cytochrome P450C11A1, cytochrome P450scc and cytochrome P450, subfamily XIA, is an enzyme that catalyzes the first step of steroid biosynthesis under the modulation of cAMP signal.
CYP11A1 in steroidogenic cells converts cholesterol to pregnenolone, which is determined by hormonal control of cholesterol availability.
Expression of the CYP11A1 gene is controlled by the transcription factor SF-1, and the upstream SF-1 binding site in the CYP11A1 gene is required for hormonal stimulation.
c-Jun and SF-1 may act synergistically to activate CYP11A1 gene expression.
Both Forskolin and 8-Br-cAMPS elevate CYP11A1 mRNA levels in the interstitial cell monolayer, which has a fully functional adenylate cyclase.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of CYP11A1. Species Crossreactivity: Human, mouse, rat
