Cyclin T1 was identified as a partner for Cdk9, an RNA polymerase II (RNAPII) transcription elongation factor.
Cyclin T1 interacts with the transactivation domain of the HIV-1 Tat protein.
The interaction of Tat with cyclin T1 enhances the affinity of Tat for the viral TAR RNA stem-loop structure, suggesting that Tat can recruit cyclin T1/cdk9 to RNAPII through cooperative binding to TAR.
The human positive transcription elongation factor b (P-TEFb) consists of a cyclin dependent kinase, cdk9, paired with a cyclin T.
Cdk9 may be paired with either cyclin T1 or cyclin T2, in a mutually exclusive manner.
Two forms of cyclin T2, T2a and T2b, are due to alternative splicing.
The binding of Tat to TAR was shown to be facilitated by human cyclin T1, but not by cyclins T2a or T2b.
Cyclin T2 binds to Cdk9 but not to Tat, and cyclin T2 can inhibit cyclin T1-mediated Tat activity.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Cyclin T1. Species Crossreactivity: Human
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant full length Human Cyclin T1.
