Cyclin H belongs to the highly conserved cyclin family, and are regulators of CDK kinases.
Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event.
Cyclin H forms a complex with CDK7 kinase and ring finger protein MAT1.
The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, and thus functions as a CDK-activating kinase (CAK).
Cyclin H and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Cyclin H. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 300-350 of Human Cyclin H.
