84-2320-69　［取扱停止］Cyclin G (Cyclin-G1, Cyclin-G, CCNG1, CCNG, CYCG1) 100ul　221984

特徴

• Cyclins are the regulatory subunits of Cdc2 p34 and related cyclin-dependent kinases (Cdks) which play critical roles in the control of cell cycle progression.
• The catalytic subunit for cyclin A and B is Cdc2 p34 kinase.
• The Cdc2- cyclin B complex controls the G2 to M transition whereas Cdc2-cyclin A regulates S phase progression.
• The G1 to S transition, however, appears to be controlled by the G1 cyclins.
• Cyclin D1 accumulates during G1 and associates with Cdk2, Cdk4 and Cdk5.
• Cyclin E and Cdk2 interact during the G1 to S transition.
• Cyclin G contains a typical N terminal cyclin box and a carboxy terminal domain sequence homologous to the tyrosine phosphorylation site of the epidermal growth factor receptor.
• Cyclin G expression is induced within 3 hours after growth stimulation and remains elevated with no apparent cell cycle dependency.
• Cyclin G2 shares 53% amino acid sequence identity with cyclin G1.
• Peak expression of cyclin G2 is seen in late S phase, as opposed to cyclin G1 expression, which is constitutive.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.