The C-X-C or alpha chemokine family is characterized by a pair of cysteine residues separated by a single amino acid and primarily functions as chemoattractants for neutrophils.
The C-X-C family includes IL-8, NAP-2, MSGA and stromal cell derived factor-1 or SDF-1.
SDF-1 was originally described as a pre-B cell stimulatory factor, but has now been shown to function as a potent chemoattractant for T cells and monocytes but not neutrophils.
Receptors for the C-X-C family are G protein coupled, seven pass transmembrane domain proteins which include IL-8RA, IL-8RB and fusin (variously referred to as LESTR or CXCR-4).
Fusin is highly homologous to the IL-8 receptors, sharing 37% sequence identity at the amino acid level.
The IL-8 receptors bind to IL-8, NAP-2 and MSGA, while fusin binds to its cognate ligand, SDF-1.
Fusin has been identified as the major coreceptor for T-tropic HIV-1 and SDF-1 has been shown to inhibit HIV-1 infection.
Applications:Suitable for use in Immunofluorescence, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of CXCR-4. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 310-360 of Human CXCR-4.
