The CXC or a chemokine family is characterized by a pair of cysteine residues separated by a single amino acid and primarily functions as chemo-attractants for neutrophils.
The CXC family includes IL-8, NAP-2, MSGA and stromal cell derived factor-1 or SDF-1.
SDF-1 was originally described as a pre-B cell stimulatory factor, but has now been shown to function as a potent chemoattractant for T cells and monocytes but not neutrophils.
Receptors for the CXC family are G protein-coupled, seven pass transmembrane domain proteins which include IL-8RA, IL-8RB, CXCR-3 and fusin (variously referred to as LESTR or CXCR-4).
CXCR-3, also known as IP-10/Mig receptor, mediates Ca++ mobilization and chemotaxis in response to the CXC chemo-kines IP-10 and Mig.
CXCR-3 is highly expressed in IL-2-activated T lymphocytes, but not in resting T lymphocytes, B lymphocytes, monocytes or granulocytes.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of CXCR-3. Species Crossreactivity: Human, mouse, rat
