Chemokines are members of a superfamily of inducible, secreted, pro-inflammatory cytokines.
Members of the chemokine family exhibit 20% to 50% homology in their predicted amino acid sequences and are divided into four subfamilies: C-C, C-X-C, C and C-X3-C.
In the C-X-C or alpha subfamily, the first two of four cysteine motifs are separated by another amino acid residue.
In the second subfamily, designated C-C or beta, the first cysteines are adjacent.
C subfamily members, also designated y chemokines, lack the first and third cysteine residues of the conserved motif.
In the C-X3-C, or delta subfamily, members have three amino acids between the two cysteines.
The C-X-C chemokine subfamily includes IL-8, GROalpha/beta/y (and the murine homologs KC, MIP-2alpha and MIP-2beta), platelet basic protein, ENA-78, GCP-2, PF4, IP-10 (and its murine homolog, CRG) and MIG.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of CXCL10. Species Crossreactivity: Human
