Chemokines are members of a superfamily of inducible,secreted, proinflammatorycytokines.
Members of the chemokine family exhibit 20-50% homologyin their predicted amino acid sequences and are divided into 4 subfamilies.
In the subfamily designated C-C or beta, the first two cysteines are adjacent.
In the C-X-C or alpha subfamily, the first two of fourcysteine residues are separated by a single amino acid.
C subfamilymembers, also designated y chemokines, lackthe first and third cysteine residues of the conserved motif.
Chemokinesin these three subfamilies are small,secreted proteins.
Fractalkine, also designated neurotactin, isthe firstcharacterizedmember of a fourth chemokine subfamily.Fractalkine contains a novel C-X3-C motif in which the first two cysteines are separated bythree amino acid residues.Fractalkine mRNA has been detected in brain and heart and is upregulated inmicroglia and endothelialcells byinflammatorysignals.
The protein can exist both as a membrane-bound formand as a chemotacticsoluble form.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of CX3CL1. Species Crossreactivity: Human
