CUG-BP1 and CUG-BP2 are proteins that bind specifically to (CUG)8 oligonucleotides in vitro.
While CUG-BP1 has the major binding activity in normal cells, nuclear CUG-BP2 binding activity increases in DM cells.
Both CUG-BP1 and CUG-BP2 are isoforms of a novel heterogeneous nuclear ribonucleoprotein (hnRNP), hNab50.
CUG-BP1, an RNA CUG triplet repeat binding protein, regulates splicing and translation of various RNAs.
Expansion of RNA CUG repeats in the DMPK in DM is associated with alterations in binding activity of CUG-BP1 as well as alterations in the translation of the C/EBPb transcription factor.
CUG-BP1 is an important regulator of initiation from different AUG codons of C/EBPb mRNA.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of CUG-BP1/2. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 62-115 of Human CUG-BP1/2.
Shelf Life：1year
Swiss Prot Number：Q92879, O95319
EU Commodity Code：30021010
商品名：CUG-BP1/2 (CUGBP Elav-like Family Member 1, CELF-1, 50kD Nuclear polyadenylated RNA-Binding Protein, Bruno-like Protein 2, CUG triplet Repeat RNA-Binding Protein 1, CUG-BP1, CUG-BP- and ETR-3-like Factor 1, Deadenylation Factor CUG-BP, Embryo deadenylation Element-Binding Protein Homolog, EDEN-BP Homolog, RNA-Binding Protein BRUNOL-2, CELF1, BRUNOL2, CUGBP, CUGBP1, NAB50, CUGBP Elav-like Family Member 2, CELF-2, Bruno-like Protein 3, CUG triplet Repeat RNA-Binding Protein 2, CUG-BP2, CUG-BP- and ETR-3-like Factor 2, ELAV-Type RNA-Binding Protein 3, ETR-3, Neuroblastoma Apoptosis-Related RNA-Binding Protein, hNAPOR, RNA-Binding Protein BRUNOL-3, CELF2, BRUNOL3, CUGBP2, ETR3, NAPOR) 50ul
