84-2319-64　［取扱停止］CtIP, phosphorylated (S327) (DNA Endonuclease RBBP8, CtBP-interacting Protein, CtIP, Retinoblastoma-Binding Protein 8, RBBP-8, Retinoblastoma-interacting Protein and Myosin-like, RIM, Sporulation in the absence of SPO11 Protein 2 Homolog, SAE2, RBBP8, CTIP) 100ul　221932

特徴

• CtBP1 is a cellular phosphoprotein that associates with various proteins and functions as a co-repressor of transcription.
• CtBP1 and the related protein CtBP2 are characterized as C-terminal binding protein of adenovirus E1A, and they preferentially associate with the E1A via a five amino acid motif, PLDLS, to repress E1A-induced oncogenesis and cellular transformation.
• CtBP1 is expressed from embryo to adult, but CtBP2 is mainly expressed during embryogenesis.
• During skeletal and T cell development, CtBP1 and CtBP2 associate with the PLDLSL domain of dEF1, a cellular zinc finger-homeodomain protein, and thereby enhance dEF1-induced transcriptional silencing.
• In addition, CtBP complexes with CtIP, a protein that recognizes distinctly different protein motifs from CtBP.
• CtIP binds to the BRCT repeats within the breast cancer gene BRCA1 and enables CtBP to influence BRCA1 activity.
• CtIP/CtBP binding to BRCA1 inhibits the transactivation of the p21 promoter, and it is critical for regulating p21 transcription in response to DNA damage.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.