Cysteine-rich proteins (CRPs) participate in the organization of multiprotein complexes, both in the cytoplasm, where they participate in cytoskeletal remodeling, and in the nucleus, where they facilitate smooth muscle differentiation.
CRP1 (cysteine and glycine-rich protein 1), also known as CRP, CSRP1 or CYRP, is abundant in the prostate and smooth muscle lineages.
It contains two LIM zinc-binding domains and is localized in the nucleus.
The LIM domains of CRP1 are critical for binding to the adhesion-plaque protein Zyxin.
CRP1 also interacts with a-actinin to mediate muscle differentiation.
These associations indicate that the main function of CRP1 may be structural.
Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunocytochemistry: 1:50-1:200Immunoprecipitation: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of CSRP1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IC IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 88-133 of Human CSRP1.
