The human IL-3, IL-5 and GM-CSF receptors are each composed of both unique a subunits and a common beta subunit.
The a subunits are low affinity ligand binding proteins while the beta subunits do not themselves bind ligand, but are required for high affinity binding by the a subunits.
In contrast, the mouse IL-3 receptor has two distinct beta subunits, one that functions only in IL-3 mediated cell signaling and a second that is shared with IL-5 and GM-CSF.
The murine beta-subunits are 91% homologous at the amino acid level but only 56% homologous to the human beta subunit.
Although neither the murine nor the human beta subunit contains tyrosine kinase domains, both activate tyrosine phosphorylation mediated signaling pathways.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, pH 7.2, 0.02% sodium azide, 50% glycerol
Specificity：Recognizes endogenous levels of human CSF2RA. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to full length human CSF2RA.
