Colony stimulating factors (CSFs) were initially characterized by their ability to stimulate in vitro colony formation by hematopoietic progenitor cells in semisolid media.
Several of these CSFs have been assigned an interleukin number, while three (GM-CSF, G-CSF and M-CSF) have retained their CSF designations.
The human granulocyte-macrophage colony stimulating factor (GM-CSF) is a pleiotropic cytokine with a 17 amino acid signal peptide that is cleaved to produce the mature form of 127 amino acids.
The mature murine GM-CSF protein is 124 amino acids and shares 60% homology with the human GM-CSF protein.
GM-CSF is a glycoprotein that can stimulate the proliferation of hematopoietic cells including granulocytes and macrophages.
It has been shown to promote the phosphorylation of cPLA2 in human neutrophils.
The phosphorylation of cPLA2 may be accompanied by an increase in enzyme activity.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of CSF2. Species Crossreactivity: Human
