alpha-crystallins consist of three gene products, alphaA, alphaB and alphaC-crystallin, which are members of the small heat shock protein family (HSP 20).
They are induced by heat shock, and act as molecular chaperones by holding denatured proteins in large soluble aggregates.
However, unlike other molecular chaperones, alpha-crystallins do not renature these proteins.
Expression of alphaA-crystallin is restricted to the lens.
Defects in this gene cause autosomal dominant congenital cataracts (ADCC).
The human alphaB-crystallin gene product is expressed in many tissues, including lens, heart and skeletal muscle.
Elevated expression of alphaB-crystallin is associated with many neurological diseases, and a missense mutation in this gene has co-segregated in a family with a Desmin-related myopathy.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Crystallin-alphaB. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 21-70 of Human Crystallin-αB.
