The adenovirus E1A protein both activates and represses gene expression to promote cellular proliferation and inhibit differentiation.
CREG (cellular repressor of E1A-stimulated genes) is a cellular protein that antagonizes transcriptional activation and cellular transformation by E1A.
CREG was initially isolated in a yeast two-hybrid screen due to its interaction with the TATA-binding protein, TBP.
Binding sites for E2F, a key transcriptional reg-ulator of cell cycle progression, are required for repression of the adeno-virus E2 promoter by CREG, and CREG was shown to inhibit activation by E2F.
CREG is broadly expressed in adult tissues and is regulated during embryonic development.
CREG is a secreted glycoprotein which enhances differentiation of mouse embryonic stem cells and human NTERA-2 cells.
CREG activity may contribute to the transcriptional control of cell growth and differentiation.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of CREG1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human CREG1.
