Aging affects oxidative metabolism in liver and other tissues.
Carnitine acyltransferases are key enzymes of this process in mitochondria.
Carnitine acetyltransferase (CAT, CRAT) catalyzes the reversible conversion of acetyl-CoA and carnitine to acetylcarnitine and CoA.
The essential functions of CAT are to regenerate CoA, which allows peroxisomal b-oxidation to proceed, and to facilitate transport of acetyl moieties to mitochondria for oxidation.
More than 70% of CAT is located in the mitochondrial matrix and it is also located in the endoplasmic reticulum, peroxisomal and mitochondrial inner membrane.
An age associated decrease in CAT activity has been reported in many rat systems.
The human gene encoding CAT maps to chromosome 9q34.1 and encodes a protein that contains a peroxisomal targeting signal and is expressed mostly in skeletal muscle, and less in heart, liver and pancreas.
Total CAT activity is induced by acetate and fatty acids, and repressed by glucose.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of CRAT. Species Crossreactivity: Human
