84-2318-25　CPT1A (Carnitine O palmitoyltransferase 1 liver Isoform, Carnitine O palmitoyltransferase I, Carnitine O palmitoyltransferase I liver Isoform, Carnitine O-palmitoyltransferase 1, Carnitine O-palmitoyltransferase I, Carnitine palmitoyltransferase 1A (liver), Carnitine palmitoyltransferase 1A, Carnitine palmitoyltransferase I, Carnitine palmitoyltransferase I liver, ...　221860

特徴

• The mitochondrial beta-oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase (CPT) I (outer membrane and detergent labile) and II (inner membrane and detergent stable), together with carnitine carrier.
• CPTI catalyzes the first reaction in the transport of long-chain fatty acids from the cytoplasm to the mitochondrion, a rate-limiting step in beta-oxidation.
• Two types of CPTI are known, the liver (CPTIA) and muscle (CPTIB) isoforms.
• The muscle type protein is specially expressed in heart and skeletal muscle.
• Membrane-bound CPTI, but not CPTII, is inhibited reversibly by malonyl-coenzyme A (CoA).
• Unlike CPTII, CPTI requires membrane integrity for catalytic function.
• In addition, glutamic acid 3 and histidine 5 are necessary for malonyl CoA inhibition and binding to liver CPTI, but not for catalytic activity.
• Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:500-1:2000Immunoprecipitation: 1:50-1:100Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.