Carboxypeptidase N (arginine carboxypeptidase or CPN) cleaves basic amino acid residues from the carboxy terminal of peptides and proteins.
The enzyme plays a central role in regulating the biologic activity of peptides such as kinins and anaphylatoxins, and therefore is also known as kininase-1 and anaphylatoxin inactivator.
CPN is a tetrameric complex consisting of two identical regulatory subunits (CPN reg) and two identical catalytic subunits (CPN cat).
The two glycosylated CPN reg subunits protect the two CPN cat subunits and keep them in the circulation.
CPN reg is a member of the leucinerich repeat family of proteins and the gene which encodes CPN reg maps to human chromosome 8p22-p23.
CPN cat is a member of the regulatory B-type carboxypeptidase group and the gene which encodes CPN cat maps to human chromosome 10.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of CPN1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 420-470 of Human CPN1.
