Prostaglandins are a diverse group of autocrine and paracrine hormones that mediate many cellular and physiologic processes.
Prostaglandin H2 (PGH2) is an intermediate in formation of the prostaglandins.
Two prostaglandin synthases that catalyze the formation of PGH2 from arachidonic acid (AA) are cyclooxygenase-1 and cyclooxygenase-2.
Cyclooxygenase-2, or Cox-2, is efficiently induced in migratory cells responding to pro-inflammatory stimuli and is considered to be an important mediator of inflammation.
An alternative form of the protein, designated Cox-1, is constitutively expressed in most tissues and is thought to serve in general “housekeeping” functions.
Both enzymes are targets for the nonsteroidal therapeutic anti-inflammatorydrugs, NSAIDs.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Cox-2. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids C-terminus of Human Cox-2.
