Cytochrome c oxidase subunit I, COX1 (also designated COI, MTCO2 or oxidative phosphorylation (OxPhos) Complex IV, subunit I) is one of three mitochondrial DNA (mtDNA) encoded subunits (MTCO1-3) of respiratory Complex IV.
Cytochrome c oxidase is a hetero-oligomeric enzyme composed of 13 subunits localized to the mitochondrial inner membrane and is the terminal enzyme complex of the electron transport chain.
Complex IV catalyzes the reduction of molecular oxygen to water.
The energy released is used to transport protons across the mitochondrial inner membrane.
The resulting electrochemical gradient is necessary for the synthesis of ATP.
Complex IV contains 13 polypeptides; COX1, COX2 and COX3 (MTCO1-3) make up the catalytic core and are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc and VIII are nuclear-encoded.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Cox-1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 555-600 of Human Cox-1.
