Catechol-O-methyltransferase (COMT) plays a crucial role in the regulation of central dopaminergic systems by catalyzing the inactivation of catechol-amines.
It is widely distributed in most tissues in soluble and membrane-bound forms.
COMT-mediated methylation metabolism of catecholamine neurotransmitters is a first-line detoxification pathway.
A Val158Met polymorphism of the COMT gene affects activity of the enzyme and influences performance and efficiency of the prefrontal cortex of the brain.
Sequential conversion of estradiol to methoxyestradiol by COMT, contributes to the antimitogenic effects of estradiol on vascular smooth muscle cell growth via estrogen receptor-independent mechanisms.
COMTD1 (catechol O-methyltransferase domain-containing protein 1) is a 262 amino acid single-pass type II membrane protein that belongs to the methyltransferase superfamily.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of COMT. Species Crossreactivity: Human, mouse, rat
