Thrombospondin 5 (also designated THBS5, cartilage oligomeric matrix protein or COMP) is the fifth member of the Thrombospondin family of extracellular matrix proteins.
The Thrombospondin family share overall homology, with significant homology in their carboxy-terminal globular domains.
They all contain type 2 (epidermal growth factor-like) and type 3 (calmodulin-like) repeats in their central domains.
The human COMP/THBS5 gene maps to chromosome 19p13.1.
Thrombospondin 5 is expressed in all types of cartilage, tendon and vascular smooth muscle.
Its localization in cartilage is developmentally regulated to the chondrocyte territorial and interterritorial matrix.
Thrombospondin 5 also binds to Collagen Type I, II and IX in a zinc-dependent manner.
Mutations in the COMP/THBS5 gene are associated with the human genetic disorders pseudoachondroplasia (PSACH) and some types of multiple epiphyseal dysplasia (MED).
PSACH and MED are autosomal dominant chondrodysplasias, which cause mild to severe short-limb dwarfism and early-onset osteoarthritis.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of COMP. Species Crossreactivity: Human
