Coilin is the primary protein of nuclear coiled (Cajal) bodies.
Cajal bodies are small nuclear organelles and contain many proteins involved in RNA transcription and processing.
Coilin is a self-associating protein with a nucleolar localization signal.
It is essential for the proper formation of Cajal bodies and for the recruitment of snRNP and survival motor neuron (SMN) complex proteins to Cajal bodies.
Coilin directly binds SMN proteins in the recruitment process and competes with SmB’ for SMN interactions.
In the developing organism, Cajal bodies play a role in the assembly of the nucleolus.
While the N-terminus of coilin contains the self-associating domain, the C-terminus of coilin regulates the number of Cajal bodies present in the cell.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of COIL. Species Crossreactivity: Human
